Thursday, July 20, 2017

How contaminated might an operating room be?

What Orthopaedic Operating Room Surfaces Are Contaminated With Bioburden? A Study Using the ATP Bioluminescence Assay

These authors asked, in their hospital which of the orthopaedic operating room surfaces were contaminated with bioburden as indicated by adenosine triphosphate (ATP) bioluminescence, which detects the energy released when ATP breaks down into adenosine biphosphate (ADP) and phosphate (P).

Six different cleaned and prepared orthopaedic operating rooms were tested before surgery with an ATP bioluminescence assay kit.  A total of 13 different surfaces were sampled once in each room. The relative light units (RLUs) obtained from each sample were recorded and data were compiled and averaged for analysis. 

All surfaces had bioburden. The highest levels were the side of the OR table headboard, computer keyboard and  tourniquet machine buttons.



Comment: While these results show that living biomaterial was present on these surfaces, the study did not correlate the ATP bioluminescence with the results of cultures to determine what types of organisms were present and specifically if these surfaces contained known pathogens. For shoulder surgeons it would have been of great interest to know if these surfaces contained Propionibacterium.
These results come from one specific hospital, and may not apply to other hospitals which would have different environments and cleaning protocols.

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